# Randomised Complete Block Anova (Stan)

This tutorial will focus on the use of Bayesian estimation to fit simple linear regression models. `BUGS`

(Bayesian inference Using *Gibbs Sampling*) is an algorithm and supporting language (resembling `R`

) dedicated to performing the Gibbs sampling implementation of *Markov Chain Monte Carlo* (MCMC) method. Dialects of the `BUGS`

language are implemented within three main projects:

**OpenBUGS**- written in component pascal.**JAGS**- (Just Another Gibbs Sampler) - written in`C++`

.**Stan**- a dedicated Bayesian modelling framework written in`C++`

and implementing*Hamiltonian*MCMC samplers.

Whilst the above programs can be used stand-alone, they do offer the rich data pre-processing and graphical capabilities of `R`

, and thus, they are best accessed from within `R`

itself. As such there are multiple packages devoted to interfacing with the various software implementations:

*R2OpenBUGS*- interfaces with`OpenBUGS`

*R2jags*- interfaces with`JAGS`

*rstan*- interfaces with`Stan`

This tutorial will demonstrate how to fit models in `Stan`

(Gelman, Lee, and Guo (2015)) using the package `rstan`

(Stan Development Team (2018)) as interface, which also requires to load some other packages.

# Overview

## Introduction

In the previous tutorial (nested ANOVA), we introduced the concept of employing sub-replicates that are nested within the main treatment levels as a means of absorbing some of the unexplained variability that would otherwise arise from designs in which sampling units are selected from amongst highly heterogeneous conditions. Such (nested) designs are useful in circumstances where the levels of the main treatment (such as burnt and un-burnt sites) occur at a much larger temporal or spatial scale than the experimental/sampling units (e.g. vegetation monitoring quadrats). For circumstances in which the main treatments can be applied (or naturally occur) at the same scale as the sampling units (such as whether a stream rock is enclosed by a fish proof fence or not), an alternative design is available. In this design (**randomised complete block design**), each of the levels of the main treatment factor are grouped (blocked) together (in space and/or time) and therefore, whilst the conditions between the groups (referred to as “blocks”) might vary substantially, the conditions under which each of the levels of the treatment are tested within any given block are far more homogeneous.

If any differences between blocks (due to the heterogeneity) can account for some of the total variability between the sampling units (thereby reducing the amount of variability that the main treatment(s) failed to explain), then the main test of treatment effects will be more powerful/sensitive. As an simple example of a randomised complete block (RCB) design, consider an investigation into the roles of different organism scales (microbial, macro invertebrate and vertebrate) on the breakdown of leaf debris packs within streams. An experiment could consist of four treatment levels - leaf packs protected by fish-proof mesh, leaf packs protected by fine macro invertebrate exclusion mesh, leaf packs protected by dissolving antibacterial tablets, and leaf packs relatively unprotected as controls. As an acknowledgement that there are many other unmeasured factors that could influence leaf pack breakdown (such as flow velocity, light levels, etc) and that these are likely to vary substantially throughout a stream, the treatments are to be arranged into groups or “blocks” (each containing a single control, microbial, macro invertebrate and fish protected leaf pack). Blocks of treatment sets are then secured in locations haphazardly selected throughout a particular reach of stream. Importantly, the arrangement of treatments in each block must be randomized to prevent the introduction of some systematic bias - such as light angle, current direction etc.

Blocking does however come at a cost. The blocks absorb both unexplained variability as well as degrees of freedom from the residuals. Consequently, if the amount of the total unexplained variation that is absorbed by the blocks is not sufficiently large enough to offset the reduction in degrees of freedom (which may result from either less than expected heterogeneity, or due to the scale at which the blocks are established being inappropriate to explain much of the variation), for a given number of sampling units (leaf packs), the tests of main treatment effects will suffer power reductions. Treatments can also be applied sequentially or repeatedly at the scale of the entire block, such that at any single time, only a single treatment level is being applied (see the lower two sub-figures above). Such designs are called repeated measures. A repeated measures ANOVA is to an single factor ANOVA as a paired t-test is to a independent samples t-test. One example of a repeated measures analysis might be an investigation into the effects of a five different diet drugs (four doses and a placebo) on the food intake of lab rats. Each of the rats (“subjects”) is subject to each of the four drugs (within subject effects) which are administered in a random order. In another example, temporal recovery responses of sharks to bi-catch entanglement stresses might be simulated by analyzing blood samples collected from captive sharks (subjects) every half hour for three hours following a stress inducing restraint. This repeated measures design allows the anticipated variability in stress tolerances between individual sharks to be accounted for in the analysis (so as to permit more powerful test of the main treatments). Furthermore, by performing repeated measures on the same subjects, repeated measures designs reduce the number of subjects required for the investigation. Essentially, this is a randomised complete block design except that the within subject (block) effect (e.g. time since stress exposure) cannot be randomised.

To suppress contamination effects resulting from the proximity of treatment sampling units within a block, units should be adequately spaced in time and space. For example, the leaf packs should not be so close to one another that the control packs are effected by the antibacterial tablets and there should be sufficient recovery time between subsequent drug administrations. In addition, the order or arrangement of treatments within the blocks must be randomized so as to prevent both confounding as well as computational complications. Whilst this is relatively straight forward for the classic randomized complete block design (such as the leaf packs in streams), it is logically not possible for repeated measures designs. Blocking factors are typically random factors that represent all the possible blocks that could be selected. As such, no individual block can truly be replicated. Randomised complete block and repeated measures designs can therefore also be thought of as un-replicated factorial designs in which there are two or more factors but that the interactions between the blocks and all the within block factors are not replicated.

## Linear models

The linear models for two and three factor nested design are:

\[ y_{ij} = \mu + \beta_i + \alpha_j + \epsilon_{ij},\]

\[ y_{ijk} = \mu + \beta_i + \alpha_j + \gamma_k + (\beta\alpha)_{ij} + (\beta\gamma)_{ik} + (\alpha\gamma)_{jk} + (\alpha\beta\gamma)_{ijk} + \epsilon_{ijk}, \;\;\; \text{(Model 1)}\]

\[ y_{ijk} = \mu + \beta_i + \alpha_j + \gamma_k + (\alpha\gamma)_{jk} + \epsilon_{ijk}, \;\;\; \text{(Model 2)},\]

where \(\mu\) is the overall mean, \(\beta\) is the effect of the Blocking Factor B (\(\sum \beta=0\)), \(\alpha\) and \(\gamma\) are the effects of withing block Factor A and Factor C, respectively, and \(\epsilon \sim N(0,\sigma^2)\) is the random unexplained or residual component.

Tests for the effects of blocks as well as effects within blocks assume that there are no interactions between blocks and the within block effects. That is, it is assumed that any effects are of similar nature within each of the blocks. Whilst this assumption may well hold for experiments that are able to consciously set the scale over which the blocking units are arranged, when designs utilize arbitrary or naturally occurring blocking units, the magnitude and even polarity of the main effects are likely to vary substantially between the blocks. The preferred (non-additive or “Model 1”) approach to un-replicated factorial analysis of some bio-statisticians is to include the block by within subject effect interactions (e.g. \(\beta\alpha\)). Whilst these interaction effects cannot be formally tested, they can be used as the denominators in F-ratio calculations of their respective main effects tests. Proponents argue that since these blocking interactions cannot be formally tested, there is no sound inferential basis for using these error terms separately. Alternatively, models can be fitted additively (“Model 2”) whereby all the block by within subject effect interactions are pooled into a single residual term (\(\epsilon\)). Although the latter approach is simpler, each of the within subject effects tests do assume that there are no interactions involving the blocks and that perhaps even more restrictively, that sphericity holds across the entire design.

## Assumptions

As with other ANOVA designs, the reliability of hypothesis tests is dependent on the residuals being:

normally distributed. Boxplots using the appropriate scale of replication (reflecting the appropriate residuals/F-ratio denominator should be used to explore normality. Scale transformations are often useful.

equally varied. Boxplots and plots of means against variance (using the appropriate scale of replication) should be used to explore the spread of values. Residual plots should reveal no patterns. Scale transformations are often useful.

independent of one another. Although the observations within a block may not strictly be independent, provided the treatments are applied or ordered randomly within each block or subject, within block proximity effects on the residuals should be random across all blocks and thus the residuals should still be independent of one another. Nevertheless, it is important that experimental units within blocks are adequately spaced in space and time so as to suppress contamination or carryover effects.

# Simple RCB

## Data generation

Imagine we has designed an experiment in which we intend to measure a response (y) to one of treatments (three levels; “a1,” “a2” and “a3”). Unfortunately, the system that we intend to sample is spatially heterogeneous and thus will add a great deal of noise to the data that will make it difficult to detect a signal (impact of treatment). Thus in an attempt to constrain this variability you decide to apply a design (RCB) in which each of the treatments within each of 35 blocks dispersed randomly throughout the landscape. As this section is mainly about the generation of artificial data (and not specifically about what to do with the data), understanding the actual details are optional and can be safely skipped.

```
> library(plyr)
> set.seed(123)
> nTreat <- 3
> nBlock <- 35
> sigma <- 5
> sigma.block <- 12
> n <- nBlock*nTreat
> Block <- gl(nBlock, k=1)
> A <- gl(nTreat,k=1)
> dt <- expand.grid(A=A,Block=Block)
> #Xmat <- model.matrix(~Block + A + Block:A, data=dt)
> Xmat <- model.matrix(~-1+Block + A, data=dt)
> block.effects <- rnorm(n = nBlock, mean = 40, sd = sigma.block)
> A.effects <- c(30,40)
> all.effects <- c(block.effects,A.effects)
> lin.pred <- Xmat %*% all.effects
>
> # OR
> Xmat <- cbind(model.matrix(~-1+Block,data=dt),model.matrix(~-1+A,data=dt))
> ## Sum to zero block effects
> block.effects <- rnorm(n = nBlock, mean = 0, sd = sigma.block)
> A.effects <- c(40,70,80)
> all.effects <- c(block.effects,A.effects)
> lin.pred <- Xmat %*% all.effects
>
>
>
> ## the quadrat observations (within sites) are drawn from
> ## normal distributions with means according to the site means
> ## and standard deviations of 5
> y <- rnorm(n,lin.pred,sigma)
> data.rcb <- data.frame(y=y, expand.grid(A=A, Block=Block))
> head(data.rcb) #print out the first six rows of the data set
y A Block
1 45.80853 1 1
2 66.71784 2 1
3 93.29238 3 1
4 43.10101 1 2
5 73.20697 2 2
6 91.77487 3 2
```

## Exploratory data analysis

**Normality and Homogeneity of variance**

`> boxplot(y~A, data.rcb)`

**Conclusions**:

there is no evidence that the response variable is consistently non-normal across all populations - each boxplot is approximately symmetrical.

there is no evidence that variance (as estimated by the height of the boxplots) differs between the five populations. . More importantly, there is no evidence of a relationship between mean and variance - the height of boxplots does not increase with increasing position along the \(y\)-axis. Hence it there is no evidence of non-homogeneity

Obvious violations could be addressed either by:

- transform the scale of the response variables (to address normality, etc). Note transformations should be applied to the entire response variable (not just those populations that are skewed).

**Block by within-Block interaction**

```
> library(car)
> with(data.rcb, interaction.plot(A,Block,y))
>
> #OR with ggplot
> library(ggplot2)
```

```
> ggplot(data.rcb, aes(y=y, x=A, group=Block,color=Block)) + geom_line() +
+ guides(color=guide_legend(ncol=3))
```

```
>
> residualPlots(lm(y~Block+A, data.rcb))
```

```
Test stat Pr(>|Test stat|)
Block
A
Tukey test -1.4163 0.1567
>
> # the Tukey's non-additivity test by itself can be obtained via an internal function
> # within the car package
> car:::tukeyNonaddTest(lm(y~Block+A, data.rcb))
Test Pvalue
-1.4163343 0.1566776
>
> # alternatively, there is also a Tukey's non-additivity test within the
> # asbio package
> library(asbio)
> with(data.rcb,tukey.add.test(y,A,Block))
Tukey's one df test for additivity
F = 2.0060029 Denom df = 67 p-value = 0.1613102
```

**Conclusions**:

- there is no visual or inferential evidence of any major interactions between Block and the within-Block effect (A). Any trends appear to be reasonably consistent between Blocks.

## Model fitting

**Full parameterisation**

\[ y_{ijk} \sim N(\mu_{ij}, \sigma^2), \;\;\; \mu_{ij}=\beta_0 + \beta_i + \gamma_{j(i)}, \]

where \(\gamma_{ij)} \sim N(0, \sigma^2_B)\), \(\beta_0, \beta_i \sim N(0, 1000000)\), and \(\sigma^2, \sigma^2_B \sim \text{Cauchy(0, 25)}\). The *full parameterisation*, shows the effects parameterisation in which there is an intercept (\(\beta_0\)) and two treatment effects (\(\beta_i\), where \(i\) is \(1,2\)).

**Matrix parameterisation**

\[ y_{ijk} \sim N(\mu_{ij}, \sigma^2), \;\;\; \mu_{ij}=\boldsymbol \beta \boldsymbol X + \gamma_{j(i)}, \]

where \(\gamma_{ij} \sim N(0, \sigma^2_B)\), \(\boldsymbol \beta \sim MVN(0, 1000000)\), and \(\sigma^2, \sigma^2_B \sim \text{Cauchy(0, 25)}\). The full parameterisation, shows the effects parameterisation in which there is an intercept (\(\alpha_0\)) and two treatment effects (\(\beta_i\), where \(i\) is \(1,2\)). The *matrix parameterisation* is a compressed notation, In this parameterisation, there are three alpha parameters (one representing the mean of treatment a1, and the other two representing the treatment effects (differences between a2 and a1 and a3 and a1). In generating priors for each of these three alpha parameters, we could loop through each and define a non-informative normal prior to each (as in the Full parameterisation version). However, it turns out that it is more efficient (in terms of mixing and thus the number of necessary iterations) to define the priors from a multivariate normal distribution. This has as many means as there are parameters to estimate (\(3\)) and a \(3\times3\) matrix of zeros and \(100\) in the diagonals.

\[ \boldsymbol \mu = \begin{bmatrix} 0 \\ 0 \\ 0 \end{bmatrix}, \;\;\; \sigma^2 \sim \begin{bmatrix} 1000000 & 0 & 0 \\ 0 & 1000000 & 0 \\ 0 & 0 & 1000000 \end{bmatrix}. \]

**Hierarchical parameterisation**

\[ y_{ijk} \sim N(\mu_{ij}, \sigma^2), \;\;\; \mu_{ij}= \beta_0 + \beta_i + \gamma_{j(i)}, \]

where \(\gamma_{ij} \sim N(0, \sigma^2_B)\), \(\beta_0, \beta_i \sim N(0, 1000000)\), and \(\sigma^2, \sigma^2_B \sim \text{Cauchy(0, 25)}\).

Rather than assume a specific variance-covariance structure, just like `lme`

we can incorporate an appropriate structure to account for different dependency/correlation structures in our data. In RCB designs, it is prudent to capture the residuals to allow checks that there are no outstanding dependency issues following model fitting.

## Full means parameterisation

```
> rstanString="
+ data{
+ int n;
+ int nA;
+ int nB;
+ vector [n] y;
+ int A[n];
+ int B[n];
+ }
+
+ parameters{
+ real alpha[nA];
+ real<lower=0> sigma;
+ vector [nB] beta;
+ real<lower=0> sigma_B;
+ }
+
+ model{
+ real mu[n];
+
+ // Priors
+ alpha ~ normal( 0 , 100 );
+ beta ~ normal( 0 , sigma_B );
+ sigma_B ~ cauchy( 0 , 25 );
+ sigma ~ cauchy( 0 , 25 );
+
+ for ( i in 1:n ) {
+ mu[i] = alpha[A[i]] + beta[B[i]];
+ }
+ y ~ normal( mu , sigma );
+ }
+
+ "
>
> ## write the model to a text file
> writeLines(rstanString, con = "fullModel.stan")
```

Arrange the data as a list (as required by `Stan`

). As input, `Stan`

will need to be supplied with: the response variable, the predictor matrix, the number of predictors, the total number of observed items. This all needs to be contained within a list object. We will create two data lists, one for each of the hypotheses.

```
> data.rcb.list <- with(data.rcb, list(y=y, A=as.numeric(A), B=as.numeric(Block),
+ n=nrow(data.rcb), nB=length(levels(Block)),nA=length(levels(A))))
```

Define the nodes (parameters and derivatives) to monitor and the chain parameters.

```
> params <- c("alpha","sigma","sigma_B")
> burnInSteps = 3000
> nChains = 2
> numSavedSteps = 3000
> thinSteps = 1
> nIter = burnInSteps+ceiling((numSavedSteps * thinSteps)/nChains)
```

Start the `Stan`

model (check the model, load data into the model, specify the number of chains and compile the model). Load the `rstan`

package.

`> library(rstan)`

Now run the `Stan`

code via the `rstan`

interface.

```
> data.rcb.rstan.c <- stan(data = data.rcb.list, file = "fullModel.stan",
+ chains = nChains, pars = params, iter = nIter,
+ warmup = burnInSteps, thin = thinSteps)
SAMPLING FOR MODEL 'fullModel' NOW (CHAIN 1).
Chain 1:
Chain 1: Gradient evaluation took 0 seconds
Chain 1: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 1: Adjust your expectations accordingly!
Chain 1:
Chain 1:
Chain 1: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 1: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 1: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 1: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 1: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 1: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 1: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 1: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 1: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 1: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 1: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 1: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 1:
Chain 1: Elapsed Time: 0.459 seconds (Warm-up)
Chain 1: 0.213 seconds (Sampling)
Chain 1: 0.672 seconds (Total)
Chain 1:
SAMPLING FOR MODEL 'fullModel' NOW (CHAIN 2).
Chain 2:
Chain 2: Gradient evaluation took 0 seconds
Chain 2: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 2: Adjust your expectations accordingly!
Chain 2:
Chain 2:
Chain 2: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 2: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 2: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 2: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 2: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 2: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 2: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 2: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 2: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 2: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 2: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 2: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 2:
Chain 2: Elapsed Time: 0.5 seconds (Warm-up)
Chain 2: 0.27 seconds (Sampling)
Chain 2: 0.77 seconds (Total)
Chain 2:
>
> print(data.rcb.rstan.c, par = c("alpha", "sigma", "sigma_B"))
Inference for Stan model: fullModel.
2 chains, each with iter=4500; warmup=3000; thin=1;
post-warmup draws per chain=1500, total post-warmup draws=3000.
mean se_mean sd 2.5% 25% 50% 75% 97.5% n_eff Rhat
alpha[1] 41.79 0.10 2.18 37.42 40.43 41.82 43.12 46.10 482 1
alpha[2] 69.70 0.10 2.18 65.25 68.32 69.76 71.12 73.84 467 1
alpha[3] 82.07 0.10 2.17 77.63 80.68 82.08 83.49 86.27 453 1
sigma 5.06 0.01 0.44 4.30 4.76 5.03 5.33 5.99 2454 1
sigma_B 11.75 0.02 1.55 9.11 10.69 11.64 12.68 15.11 4039 1
Samples were drawn using NUTS(diag_e) at Thu Jul 08 20:48:56 2021.
For each parameter, n_eff is a crude measure of effective sample size,
and Rhat is the potential scale reduction factor on split chains (at
convergence, Rhat=1).
>
> data.rcb.rstan.c.df <-as.data.frame(extract(data.rcb.rstan.c))
> head(data.rcb.rstan.c.df)
alpha.1 alpha.2 alpha.3 sigma sigma_B lp__
1 42.57143 72.31562 82.21306 4.443121 14.22092 -318.3567
2 44.04634 72.80428 82.79750 5.358441 12.36527 -332.7155
3 37.28553 65.33775 76.80241 5.776490 12.95970 -327.7117
4 40.86545 67.75901 81.25944 5.250018 11.54635 -320.7851
5 42.24118 69.79130 81.13336 5.684114 11.53545 -323.1236
6 46.50718 72.92837 86.52039 5.017633 11.35059 -324.5646
>
> data.rcb.mcmc.c<-rstan:::as.mcmc.list.stanfit(data.rcb.rstan.c)
>
> library(coda)
> MCMCsum <- function(x) {
+ data.frame(Median=median(x, na.rm=TRUE), t(quantile(x,na.rm=TRUE)),
+ HPDinterval(as.mcmc(x)),HPDinterval(as.mcmc(x),p=0.5))
+ }
>
> plyr:::adply(as.matrix(data.rcb.rstan.c.df),2,MCMCsum)
X1 Median X0. X25. X50. X75.
1 alpha.1 41.820028 33.575693 40.428322 41.820028 43.116642
2 alpha.2 69.755101 61.468709 68.319038 69.755101 71.122032
3 alpha.3 82.079951 71.625677 80.680363 82.079951 83.486135
4 sigma 5.026758 3.750734 4.758087 5.026758 5.333697
5 sigma_B 11.638484 7.483196 10.685479 11.638484 12.682813
6 lp__ -321.508304 -345.176879 -325.030733 -321.508304 -318.271715
X100. lower upper lower.1 upper.1
1 48.718666 37.373165 46.031150 40.451754 43.127117
2 76.610258 65.314489 73.863166 68.275779 71.073703
3 89.330603 77.963087 86.521250 80.526700 83.288573
4 7.005605 4.282499 5.965741 4.665417 5.228678
5 19.655896 8.767018 14.666714 10.665432 12.653049
6 -307.513824 -333.407865 -312.343598 -324.913583 -318.188585
```

## Full effect parameterisation

```
> rstan2String="
+ data{
+ int n;
+ int nB;
+ vector [n] y;
+ int A2[n];
+ int A3[n];
+ int B[n];
+ }
+
+ parameters{
+ real alpha0;
+ real alpha2;
+ real alpha3;
+ real<lower=0> sigma;
+ vector [nB] beta;
+ real<lower=0> sigma_B;
+ }
+
+ model{
+ real mu[n];
+
+ // Priors
+ alpha0 ~ normal( 0 , 1000 );
+ alpha2 ~ normal( 0 , 1000 );
+ alpha3 ~ normal( 0 , 1000 );
+ beta ~ normal( 0 , sigma_B );
+ sigma_B ~ cauchy( 0 , 25 );
+ sigma ~ cauchy( 0 , 25 );
+
+ for ( i in 1:n ) {
+ mu[i] = alpha0 + alpha2*A2[i] +
+ alpha3*A3[i] + beta[B[i]];
+ }
+ y ~ normal( mu , sigma );
+ }
+
+ "
>
> ## write the model to a text file
> writeLines(rstan2String, con = "full2Model.stan")
```

Arrange the data as a list (as required by `Stan`

). As input, `Stan`

will need to be supplied with: the response variable, the predictor matrix, the number of predictors, the total number of observed items. This all needs to be contained within a list object. We will create two data lists, one for each of the hypotheses.

```
> A2 <- ifelse(data.rcb$A=='2',1,0)
> A3 <- ifelse(data.rcb$A=='3',1,0)
> data.rcb.list <- with(data.rcb, list(y=y, A2=A2, A3=A3, B=as.numeric(Block),
+ n=nrow(data.rcb), nB=length(levels(Block))))
```

Define the nodes (parameters and derivatives) to monitor and the chain parameters.

```
> params <- c("alpha0","alpha2","alpha3","sigma","sigma_B")
> burnInSteps = 3000
> nChains = 2
> numSavedSteps = 3000
> thinSteps = 1
> nIter = burnInSteps+ceiling((numSavedSteps * thinSteps)/nChains)
```

Now run the `Stan`

code via the `rstan`

interface.

```
> data.rcb.rstan.f <- stan(data = data.rcb.list, file = "full2Model.stan",
+ chains = nChains, pars = params, iter = nIter,
+ warmup = burnInSteps, thin = thinSteps)
SAMPLING FOR MODEL 'full2Model' NOW (CHAIN 1).
Chain 1:
Chain 1: Gradient evaluation took 0.001 seconds
Chain 1: 1000 transitions using 10 leapfrog steps per transition would take 10 seconds.
Chain 1: Adjust your expectations accordingly!
Chain 1:
Chain 1:
Chain 1: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 1: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 1: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 1: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 1: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 1: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 1: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 1: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 1: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 1: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 1: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 1: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 1:
Chain 1: Elapsed Time: 1.203 seconds (Warm-up)
Chain 1: 0.456 seconds (Sampling)
Chain 1: 1.659 seconds (Total)
Chain 1:
SAMPLING FOR MODEL 'full2Model' NOW (CHAIN 2).
Chain 2:
Chain 2: Gradient evaluation took 0 seconds
Chain 2: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 2: Adjust your expectations accordingly!
Chain 2:
Chain 2:
Chain 2: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 2: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 2: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 2: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 2: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 2: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 2: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 2: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 2: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 2: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 2: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 2: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 2:
Chain 2: Elapsed Time: 1.208 seconds (Warm-up)
Chain 2: 0.448 seconds (Sampling)
Chain 2: 1.656 seconds (Total)
Chain 2:
>
> print(data.rcb.rstan.f, par = c("alpha0", "alpha2", "alpha3", "sigma", "sigma_B"))
Inference for Stan model: full2Model.
2 chains, each with iter=4500; warmup=3000; thin=1;
post-warmup draws per chain=1500, total post-warmup draws=3000.
mean se_mean sd 2.5% 25% 50% 75% 97.5% n_eff Rhat
alpha0 41.73 0.12 2.06 37.70 40.31 41.73 43.14 45.77 279 1.01
alpha2 27.98 0.03 1.26 25.52 27.10 28.00 28.83 30.39 2337 1.00
alpha3 40.31 0.03 1.28 37.87 39.43 40.27 41.20 42.86 2342 1.00
sigma 5.08 0.01 0.45 4.30 4.77 5.05 5.36 6.09 1996 1.00
sigma_B 11.71 0.03 1.54 9.16 10.63 11.54 12.59 15.28 2753 1.00
Samples were drawn using NUTS(diag_e) at Thu Jul 08 20:49:26 2021.
For each parameter, n_eff is a crude measure of effective sample size,
and Rhat is the potential scale reduction factor on split chains (at
convergence, Rhat=1).
>
> data.rcb.rstan.f.df <-as.data.frame(extract(data.rcb.rstan.f))
> head(data.rcb.rstan.f.df)
alpha0 alpha2 alpha3 sigma sigma_B lp__
1 39.22545 28.90767 41.24224 5.307120 10.07145 -319.8347
2 43.93555 28.00887 41.30383 5.131755 13.61273 -320.8549
3 42.54761 28.77128 38.96852 4.831529 10.79286 -321.2491
4 40.29869 27.77761 41.45388 5.268648 12.51140 -321.3806
5 39.80535 27.62258 40.84395 5.610155 11.92872 -322.8471
6 44.79405 27.48598 40.33440 4.957009 10.57214 -315.7301
>
> data.rcb.mcmc.f<-rstan:::as.mcmc.list.stanfit(data.rcb.rstan.f)
>
> plyr:::adply(as.matrix(data.rcb.rstan.f.df),2,MCMCsum)
X1 Median X0. X25. X50. X75.
1 alpha0 41.725509 34.892539 40.308333 41.725509 43.140187
2 alpha2 27.996917 23.328561 27.104569 27.996917 28.825079
3 alpha3 40.270625 35.879999 39.434168 40.270625 41.199753
4 sigma 5.047603 3.962453 4.770682 5.047603 5.358117
5 sigma_B 11.541455 7.826250 10.627376 11.541455 12.590550
6 lp__ -321.225937 -341.591496 -324.985012 -321.225937 -317.730035
X100. lower upper lower.1 upper.1
1 48.60702 37.86766 45.820530 40.519110 43.34783
2 32.25281 25.67172 30.496226 27.195515 28.88708
3 44.93039 38.04721 42.996236 39.526151 41.27658
4 7.09570 4.20633 5.958481 4.713769 5.29742
5 19.35289 9.00899 14.880664 10.415028 12.30244
6 -306.67832 -331.55239 -310.780917 -323.954151 -316.83537
```

## Matrix parameterisation

```
> rstanString2="
+ data{
+ int n;
+ int nX;
+ int nB;
+ vector [n] y;
+ matrix [n,nX] X;
+ int B[n];
+ }
+
+ parameters{
+ vector [nX] beta;
+ real<lower=0> sigma;
+ vector [nB] gamma;
+ real<lower=0> sigma_B;
+ }
+ transformed parameters {
+ vector[n] mu;
+
+ mu = X*beta;
+ for (i in 1:n) {
+ mu[i] = mu[i] + gamma[B[i]];
+ }
+ }
+ model{
+ // Priors
+ beta ~ normal( 0 , 100 );
+ gamma ~ normal( 0 , sigma_B );
+ sigma_B ~ cauchy( 0 , 25 );
+ sigma ~ cauchy( 0 , 25 );
+
+ y ~ normal( mu , sigma );
+ }
+
+ "
>
> ## write the model to a text file
> writeLines(rstanString2, con = "matrixModel.stan")
```

Arrange the data as a list (as required by `Stan`

). As input, `Stan`

will need to be supplied with: the response variable, the predictor matrix, the number of predictors, the total number of observed items. This all needs to be contained within a list object. We will create two data lists, one for each of the hypotheses.

```
> Xmat <- model.matrix(~A, data=data.rcb)
> data.rcb.list <- with(data.rcb, list(y=y, X=Xmat, nX=ncol(Xmat),
+ B=as.numeric(Block),
+ n=nrow(data.rcb), nB=length(levels(Block))))
```

Define the nodes (parameters and derivatives) to monitor and the chain parameters.

```
> params <- c("beta","sigma","sigma_B")
> burnInSteps = 3000
> nChains = 2
> numSavedSteps = 3000
> thinSteps = 1
> nIter = burnInSteps+ceiling((numSavedSteps * thinSteps)/nChains)
```

Now run the `Stan`

code via the `rstan`

interface.

```
> data.rcb.rstan.d <- stan(data = data.rcb.list, file = "matrixModel.stan",
+ chains = nChains, pars = params, iter = nIter,
+ warmup = burnInSteps, thin = thinSteps)
SAMPLING FOR MODEL 'matrixModel' NOW (CHAIN 1).
Chain 1:
Chain 1: Gradient evaluation took 0 seconds
Chain 1: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 1: Adjust your expectations accordingly!
Chain 1:
Chain 1:
Chain 1: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 1: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 1: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 1: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 1: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 1: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 1: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 1: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 1: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 1: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 1: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 1: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 1:
Chain 1: Elapsed Time: 0.696 seconds (Warm-up)
Chain 1: 0.278 seconds (Sampling)
Chain 1: 0.974 seconds (Total)
Chain 1:
SAMPLING FOR MODEL 'matrixModel' NOW (CHAIN 2).
Chain 2:
Chain 2: Gradient evaluation took 0 seconds
Chain 2: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 2: Adjust your expectations accordingly!
Chain 2:
Chain 2:
Chain 2: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 2: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 2: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 2: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 2: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 2: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 2: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 2: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 2: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 2: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 2: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 2: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 2:
Chain 2: Elapsed Time: 0.748 seconds (Warm-up)
Chain 2: 0.268 seconds (Sampling)
Chain 2: 1.016 seconds (Total)
Chain 2:
>
> print(data.rcb.rstan.d, par = c("beta", "sigma", "sigma_B"))
Inference for Stan model: matrixModel.
2 chains, each with iter=4500; warmup=3000; thin=1;
post-warmup draws per chain=1500, total post-warmup draws=3000.
mean se_mean sd 2.5% 25% 50% 75% 97.5% n_eff Rhat
beta[1] 41.74 0.13 2.14 37.61 40.30 41.74 43.13 45.96 283 1
beta[2] 27.96 0.02 1.19 25.60 27.18 27.96 28.76 30.33 2981 1
beta[3] 40.29 0.02 1.19 37.99 39.47 40.29 41.08 42.66 2664 1
sigma 5.06 0.01 0.45 4.28 4.74 5.03 5.34 6.01 1923 1
sigma_B 11.71 0.03 1.56 9.17 10.58 11.55 12.65 15.06 2405 1
Samples were drawn using NUTS(diag_e) at Thu Jul 08 20:49:54 2021.
For each parameter, n_eff is a crude measure of effective sample size,
and Rhat is the potential scale reduction factor on split chains (at
convergence, Rhat=1).
>
> data.rcb.rstan.d.df <-as.data.frame(extract(data.rcb.rstan.d))
> head(data.rcb.rstan.d.df)
beta.1 beta.2 beta.3 sigma sigma_B lp__
1 41.89677 27.16321 40.47042 5.245901 16.83721 -321.1680
2 40.45193 28.26549 39.52228 4.848226 11.45847 -319.1260
3 41.31717 27.69851 40.54105 5.191861 10.36840 -310.0800
4 42.38571 28.61291 39.06232 5.708599 14.45468 -332.7570
5 43.77131 27.65950 41.95679 5.061046 13.05687 -315.3818
6 40.34171 27.42241 41.72770 5.093326 10.22403 -320.9606
>
> data.rcb.mcmc.d<-rstan:::as.mcmc.list.stanfit(data.rcb.rstan.d)
>
> plyr:::adply(as.matrix(data.rcb.rstan.d.df),2,MCMCsum)
X1 Median X0. X25. X50. X75.
1 beta.1 41.736756 33.482760 40.302747 41.736756 43.13230
2 beta.2 27.964371 23.330623 27.178174 27.964371 28.75517
3 beta.3 40.287410 35.920567 39.468235 40.287410 41.08209
4 sigma 5.029576 3.801037 4.740091 5.029576 5.33871
5 sigma_B 11.550917 7.321285 10.584764 11.550917 12.64664
6 lp__ -321.010597 -344.389988 -324.976499 -321.010597 -317.79906
X100. lower upper lower.1 upper.1
1 48.291349 37.436094 45.719579 40.182154 42.995630
2 32.305073 25.565749 30.256798 27.138701 28.682175
3 44.616108 37.952774 42.596752 39.454405 41.049342
4 7.270289 4.224055 5.913362 4.711782 5.301598
5 19.699834 9.076929 14.849617 10.284478 12.264297
6 -306.943361 -332.094979 -311.576717 -323.851762 -316.850881
```

# RCB (repeated measures) - continuous within

## Data generation

Imagine now that we has designed an experiment to investigate the effects of a continuous predictor (\(x\), for example time) on a response (\(y\)). Again, the system that we intend to sample is spatially heterogeneous and thus will add a great deal of noise to the data that will make it difficult to detect a signal (impact of treatment). Thus in an attempt to constrain this variability, we again decide to apply a design (RCB) in which each of the levels of \(X\) (such as time) treatments within each of \(35\) blocks dispersed randomly throughout the landscape. As this section is mainly about the generation of artificial data (and not specifically about what to do with the data), understanding the actual details are optional and can be safely skipped.

```
> set.seed(123)
> slope <- 30
> intercept <- 200
> nBlock <- 35
> nTime <- 10
> sigma <- 50
> sigma.block <- 30
> n <- nBlock*nTime
> Block <- gl(nBlock, k=1)
> Time <- 1:10
> rho <- 0.8
> dt <- expand.grid(Time=Time,Block=Block)
> Xmat <- model.matrix(~-1+Block + Time, data=dt)
> block.effects <- rnorm(n = nBlock, mean = intercept, sd = sigma.block)
> #A.effects <- c(30,40)
> all.effects <- c(block.effects,slope)
> lin.pred <- Xmat %*% all.effects
>
> # OR
> Xmat <- cbind(model.matrix(~-1+Block,data=dt),model.matrix(~Time,data=dt))
> ## Sum to zero block effects
> ##block.effects <- rnorm(n = nBlock, mean = 0, sd = sigma.block)
> ###A.effects <- c(40,70,80)
> ##all.effects <- c(block.effects,intercept,slope)
> ##lin.pred <- Xmat %*% all.effects
>
> ## the quadrat observations (within sites) are drawn from
> ## normal distributions with means according to the site means
> ## and standard deviations of 5
> eps <- NULL
> eps[1] <- 0
> for (j in 2:n) {
+ eps[j] <- rho*eps[j-1] #residuals
+ }
> y <- rnorm(n,lin.pred,sigma)+eps
>
> #OR
> eps <- NULL
> # first value cant be autocorrelated
> eps[1] <- rnorm(1,0,sigma)
> for (j in 2:n) {
+ eps[j] <- rho*eps[j-1] + rnorm(1, mean = 0, sd = sigma) #residuals
+ }
> y <- lin.pred + eps
> data.rm <- data.frame(y=y, dt)
> head(data.rm) #print out the first six rows of the data set
y Time Block
1 282.1142 1 1
2 321.1404 2 1
3 278.7700 3 1
4 285.8709 4 1
5 336.6390 5 1
6 333.5961 6 1
>
> ggplot(data.rm, aes(y=y, x=Time)) + geom_smooth(method='lm') + geom_point() + facet_wrap(~Block)
```

## Exploratory data analysis

**Normality and Homogeneity of variance**

`> boxplot(y~Time, data.rm)`

```
>
> ggplot(data.rm, aes(y=y, x=factor(Time))) + geom_boxplot()
```

**Conclusions**:

there is no evidence that the response variable is consistently non-normal across all populations - each boxplot is approximately symmetrical.

there is no evidence that variance (as estimated by the height of the boxplots) differs between the five populations. More importantly, there is no evidence of a relationship between mean and variance - the height of boxplots does not increase with increasing position along the \(y\)-axis. Hence it there is no evidence of non-homogeneity

Obvious violations could be addressed either by:

- transform the scale of the response variables (to address normality, etc). Note transformations should be applied to the entire response variable (not just those populations that are skewed).

**Block by within-Block interaction**

`> with(data.rm, interaction.plot(Time,Block,y))`

```
>
> ggplot(data.rm, aes(y=y, x=Time, color=Block, group=Block)) + geom_line() +
+ guides(color=guide_legend(ncol=3))
```

```
>
> residualPlots(lm(y~Block+Time, data.rm))
```

```
Test stat Pr(>|Test stat|)
Block
Time -0.7274 0.4675
Tukey test -0.9809 0.3267
>
> # the Tukey's non-additivity test by itself can be obtained via an internal function
> # within the car package
> car:::tukeyNonaddTest(lm(y~Block+Time, data.rm))
Test Pvalue
-0.9808606 0.3266615
>
> # alternatively, there is also a Tukey's non-additivity test within the
> # asbio package
> with(data.rm,tukey.add.test(y,Time,Block))
Tukey's one df test for additivity
F = 0.3997341 Denom df = 305 p-value = 0.5277003
```

**Conclusions**:

- there is no visual or inferential evidence of any major interactions between Block and the within-Block effect (Time). Any trends appear to be reasonably consistent between Blocks.

**Sphericity**

Since the levels of Time cannot be randomly assigned, it is likely that sphericity is not met. We can explore whether there is an auto-correlation patterns in the residuals. Note, as there was only ten time periods, it does not make logical sense to explore lags above \(10\).

```
> library(nlme)
> data.rm.lme <- lme(y~Time, random=~1|Block, data=data.rm)
> acf(resid(data.rm.lme), lag=10)
```

**Conclusions**:

The autocorrelation factor (ACF) at a range of lags up to \(10\), indicate that there is a cyclical pattern of residual auto-correlation. We really should explore incorporating some form of correlation structure into our model.

## Model fitting

## Matrix parameterisation

```
> rstanString2="
+ data{
+ int n;
+ int nX;
+ int nB;
+ vector [n] y;
+ matrix [n,nX] X;
+ int B[n];
+ }
+
+ parameters{
+ vector [nX] beta;
+ real<lower=0> sigma;
+ vector [nB] gamma;
+ real<lower=0> sigma_B;
+ }
+ transformed parameters {
+ vector[n] mu;
+
+ mu = X*beta;
+ for (i in 1:n) {
+ mu[i] = mu[i] + gamma[B[i]];
+ }
+ }
+ model{
+ // Priors
+ beta ~ normal( 0 , 100 );
+ gamma ~ normal( 0 , sigma_B );
+ sigma_B ~ cauchy( 0 , 25 );
+ sigma ~ cauchy( 0 , 25 );
+
+ y ~ normal( mu , sigma );
+ }
+
+ "
>
> ## write the model to a text file
> writeLines(rstanString2, con = "matrixModel2.stan")
>
> Xmat <- model.matrix(~Time, data=data.rm)
> data.rm.list <- with(data.rm, list(y=y, X=Xmat, nX=ncol(Xmat),
+ B=as.numeric(Block),
+ n=nrow(data.rm), nB=length(levels(Block))))
>
> params <- c('beta','sigma','sigma_B')
> burnInSteps = 3000
> nChains = 2
> numSavedSteps = 3000
> thinSteps = 1
> nIter = burnInSteps+ceiling((numSavedSteps * thinSteps)/nChains)
>
> data.rm.rstan.d <- stan(data = data.rm.list, file = "matrixModel2.stan",
+ chains = nChains, pars = params, iter = nIter,
+ warmup = burnInSteps, thin = thinSteps)
SAMPLING FOR MODEL 'matrixModel' NOW (CHAIN 1).
Chain 1:
Chain 1: Gradient evaluation took 0 seconds
Chain 1: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 1: Adjust your expectations accordingly!
Chain 1:
Chain 1:
Chain 1: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 1: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 1: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 1: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 1: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 1: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 1: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 1: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 1: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 1: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 1: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 1: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 1:
Chain 1: Elapsed Time: 2.103 seconds (Warm-up)
Chain 1: 0.579 seconds (Sampling)
Chain 1: 2.682 seconds (Total)
Chain 1:
SAMPLING FOR MODEL 'matrixModel' NOW (CHAIN 2).
Chain 2:
Chain 2: Gradient evaluation took 0 seconds
Chain 2: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 2: Adjust your expectations accordingly!
Chain 2:
Chain 2:
Chain 2: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 2: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 2: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 2: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 2: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 2: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 2: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 2: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 2: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 2: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 2: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 2: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 2:
Chain 2: Elapsed Time: 2.437 seconds (Warm-up)
Chain 2: 0.562 seconds (Sampling)
Chain 2: 2.999 seconds (Total)
Chain 2:
>
> print(data.rm.rstan.d , par = c('beta','sigma','sigma_B'))
Inference for Stan model: matrixModel.
2 chains, each with iter=4500; warmup=3000; thin=1;
post-warmup draws per chain=1500, total post-warmup draws=3000.
mean se_mean sd 2.5% 25% 50% 75% 97.5% n_eff Rhat
beta[1] 187.47 0.69 12.66 162.92 178.74 187.42 196.05 212.47 333 1.01
beta[2] 30.79 0.02 1.04 28.69 30.11 30.79 31.49 32.83 2219 1.00
sigma 55.83 0.05 2.29 51.50 54.27 55.76 57.34 60.56 2549 1.00
sigma_B 64.64 0.18 8.63 50.05 58.48 63.76 69.76 84.33 2346 1.00
Samples were drawn using NUTS(diag_e) at Thu Jul 08 20:50:04 2021.
For each parameter, n_eff is a crude measure of effective sample size,
and Rhat is the potential scale reduction factor on split chains (at
convergence, Rhat=1).
```

Given that Time cannot be randomized, there is likely to be a temporal dependency structure to the data. The above analyses assume no temporal dependency - actually, they assume that the variance-covariance matrix demonstrates a structure known as sphericity. Lets specifically model in a first order autoregressive correlation structure in an attempt to accommodate the expected temporal autocorrelation.

```
> rstanString3="
+ data{
+ int n;
+ int nX;
+ int nB;
+ vector [n] y;
+ matrix [n,nX] X;
+ int B[n];
+ vector [n] tgroup;
+ }
+
+ parameters{
+ vector [nX] beta;
+ real<lower=0> sigma;
+ vector [nB] gamma;
+ real<lower=0> sigma_B;
+ real ar;
+ }
+ transformed parameters {
+ vector[n] mu;
+ vector[n] E;
+ vector[n] res;
+
+ mu = X*beta;
+ for (i in 1:n) {
+ E[i] = 0;
+ }
+ for (i in 1:n) {
+ mu[i] = mu[i] + gamma[B[i]];
+ res[i] = y[i] - mu[i];
+ if(i>0 && i < n && tgroup[i+1] == tgroup[i]) {
+ E[i+1] = res[i];
+ }
+ mu[i] = mu[i] + (E[i] * ar);
+ }
+ }
+ model{
+ // Priors
+ beta ~ normal( 0 , 100 );
+ gamma ~ normal( 0 , sigma_B );
+ sigma_B ~ cauchy( 0 , 25 );
+ sigma ~ cauchy( 0 , 25 );
+
+ y ~ normal( mu , sigma );
+ }
+
+ "
>
> ## write the model to a text file
> writeLines(rstanString3, con = "matrixModel3.stan")
>
> Xmat <- model.matrix(~Time, data=data.rm)
> data.rm.list <- with(data.rm, list(y=y, X=Xmat, nX=ncol(Xmat),
+ B=as.numeric(Block),
+ n=nrow(data.rm), nB=length(levels(Block)),
+ tgroup=as.numeric(Block)))
>
> params <- c('beta','sigma','sigma_B','ar')
> burnInSteps = 3000
> nChains = 2
> numSavedSteps = 3000
> thinSteps = 1
> nIter = burnInSteps+ceiling((numSavedSteps * thinSteps)/nChains)
>
> data.rm.rstan.d <- stan(data = data.rm.list, file = "matrixModel3.stan",
+ chains = nChains, pars = params, iter = nIter,
+ warmup = burnInSteps, thin = thinSteps)
SAMPLING FOR MODEL 'matrixModel3' NOW (CHAIN 1).
Chain 1:
Chain 1: Gradient evaluation took 0 seconds
Chain 1: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 1: Adjust your expectations accordingly!
Chain 1:
Chain 1:
Chain 1: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 1: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 1: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 1: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 1: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 1: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 1: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 1: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 1: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 1: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 1: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 1: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 1:
Chain 1: Elapsed Time: 6.562 seconds (Warm-up)
Chain 1: 1.678 seconds (Sampling)
Chain 1: 8.24 seconds (Total)
Chain 1:
SAMPLING FOR MODEL 'matrixModel3' NOW (CHAIN 2).
Chain 2:
Chain 2: Gradient evaluation took 0 seconds
Chain 2: 1000 transitions using 10 leapfrog steps per transition would take 0 seconds.
Chain 2: Adjust your expectations accordingly!
Chain 2:
Chain 2:
Chain 2: Iteration: 1 / 4500 [ 0%] (Warmup)
Chain 2: Iteration: 450 / 4500 [ 10%] (Warmup)
Chain 2: Iteration: 900 / 4500 [ 20%] (Warmup)
Chain 2: Iteration: 1350 / 4500 [ 30%] (Warmup)
Chain 2: Iteration: 1800 / 4500 [ 40%] (Warmup)
Chain 2: Iteration: 2250 / 4500 [ 50%] (Warmup)
Chain 2: Iteration: 2700 / 4500 [ 60%] (Warmup)
Chain 2: Iteration: 3001 / 4500 [ 66%] (Sampling)
Chain 2: Iteration: 3450 / 4500 [ 76%] (Sampling)
Chain 2: Iteration: 3900 / 4500 [ 86%] (Sampling)
Chain 2: Iteration: 4350 / 4500 [ 96%] (Sampling)
Chain 2: Iteration: 4500 / 4500 [100%] (Sampling)
Chain 2:
Chain 2: Elapsed Time: 13.585 seconds (Warm-up)
Chain 2: 1.878 seconds (Sampling)
Chain 2: 15.463 seconds (Total)
Chain 2:
>
> print(data.rm.rstan.d , par = c('beta','sigma','sigma_B','ar'))
Inference for Stan model: matrixModel3.
2 chains, each with iter=4500; warmup=3000; thin=1;
post-warmup draws per chain=1500, total post-warmup draws=3000.
mean se_mean sd 2.5% 25% 50% 75% 97.5% n_eff Rhat
beta[1] 179.47 0.25 12.40 155.58 171.19 179.51 187.90 203.08 2542 1
beta[2] 31.32 0.02 1.66 28.06 30.16 31.31 32.44 34.51 5078 1
sigma 48.75 0.04 2.05 45.00 47.31 48.67 50.10 53.01 2856 1
sigma_B 49.71 0.36 10.78 30.30 42.47 49.27 56.72 72.54 879 1
ar 0.78 0.00 0.05 0.68 0.75 0.78 0.82 0.88 2296 1
Samples were drawn using NUTS(diag_e) at Thu Jul 08 20:50:56 2021.
For each parameter, n_eff is a crude measure of effective sample size,
and Rhat is the potential scale reduction factor on split chains (at
convergence, Rhat=1).
```

# References

*Journal of Educational and Behavioral Statistics*40 (5): 530–43.